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rat igg 2b isotype control antibody  (R&D Systems)


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    R&D Systems rat igg 2b isotype control antibody
    Rat Igg 2b Isotype Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+igg2b+isotype+control+antibodies/Rat+IgG2B+Isotype+Control/bio_rxiv__64898__2026__01__27__701820-204-16-24
    Average 93 stars, based on 153 article reviews
    rat igg 2b isotype control antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Control:

    Article Title: Anti-ST2 antibody reduces airway hyperresponsiveness mediated by monocyte-derived macrophages during influenza A infection.
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) of BAL and lung homogenate supernatants using mouse DuoSet ELISA kits for IL-33, IL-13, CCL2 (R&D Systems), express cysteinyl leukotrienes (CysLTs) (Cayman Chemical, Ann Arbor, MI, USA) and serotonin (Abcam, Cambridge, UK) were conducted according to the manufacturer’s instructions. .. Anti-ST2 and rat IgG2B isotype control antibodies (R&D Systems, Minneapolis, Minnesota, USA), 10 μg each in sterile PBS, were delivered intranasally a day before and a day following sub lethal IAV infection under light anaesthesia[27]. ..

    Article Title: Methods for detecting and treating cancer
    Article Snippet: .. Anti-podocalyxin (clone 192703) and rat IgG2b isotype control antibodies were purchased from R&D Systems (Minneapolis, Minn.). .. All other antibodies used were from commercial sources including anti-α4 integrin, Ter119-PE, c-Kit and PECAM (BD Pharmingen, Mississauga, ON) and β1-integrin (BD Transduction Labs).

    Article Title: The IKK?-dependent NF-?B p52/RelB non-canonical pathway is essential to sustain a CXCL12 autocrine loop in cells migrating in response to HMGB1
    Article Snippet: Recombinant murine SDF-1) was from PeproTech (Rocky Hill, NJ). .. Other reagents were obtained from the following suppliers: 8 μm pore size cellulose nitrate filters (for macrophages) and PVP-free polycarbonate filters (for fibroblasts) for 48 well microchemotaxis (Boyden-type) chambers (Neuroprobe Inc., Cabin John, MD); fibronectin (Roche), human recombinant PDGF (R&D Systems, Minneapolis, MN); Human recombinant complement C5a, 4-hydroxytamoxifen (4-OHT), AMD3100 (Sigma, St. Louis, MO), alexa flour 488 conjugated streptavidin (Molecular probes, Eugene, OR), alexa flour 647 conjugated anti-CXCR4 antibody (BioLegend) and biotin conjugated anti-RAGE antibody, anti-CXCR4 neutralizing monoclonal antibody, mouse IgG2a and rat IgG2b isotype control antibodies (all from R&D systems). ..

    Article Title: Longitudinal changes in the expression of IL-33 and IL-33 regulated genes in relapsing remitting MS
    Article Snippet: Cells were then washed twice with 0.75 ml of ice-cold perm/wash solution (BD Biosciences, San Diego, CA) at 3000 rpm for 5 min each. .. After washing, cells were incubated with 3 μl of PE conjugated rat (IgG2B) anti-human IL-33 (R&D systems; cat # IC3625P; Minneapolis, MN) antibodies in 150 μl volume of perm wash buffer at room temperature for 1 h. Part of the unstained and CD14 stained cells were also stained with rat (IgG2B) isotype control antibodies (R&D systems; cat # IC013P; Minneapolis, MN) in 150 μl volume of perm wash buffer at room temperature for 1 h. Cells were then washed twice with 0.75 ml of ice-cold perm/wash solution and re-suspended in 0.5 ml of PBS for flow cytometry analysis. shows the flow cytometric distribution of CD14+ IL-33+ cells. ..

    Article Title: Heart-resident CCR2 + macrophages promote neutrophil extravasation through TLR9/MyD88/CXCL5 signaling
    Article Snippet: CCR2-expressing cells were depleted in donor hearts by injecting DT (250 ng i.p. 3, 2, and 1 days prior to heart harvest; Sigma-Aldrich) into CCR2-DTR mice. .. Some recipient mice were treated with CXCL2-neutraliizing or rat IgG2B isotype control antibodies (200 μg i.v. at the time of reperfusion, R&D Systems). ..

    Sterility:

    Article Title: Anti-ST2 antibody reduces airway hyperresponsiveness mediated by monocyte-derived macrophages during influenza A infection.
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) of BAL and lung homogenate supernatants using mouse DuoSet ELISA kits for IL-33, IL-13, CCL2 (R&D Systems), express cysteinyl leukotrienes (CysLTs) (Cayman Chemical, Ann Arbor, MI, USA) and serotonin (Abcam, Cambridge, UK) were conducted according to the manufacturer’s instructions. .. Anti-ST2 and rat IgG2B isotype control antibodies (R&D Systems, Minneapolis, Minnesota, USA), 10 μg each in sterile PBS, were delivered intranasally a day before and a day following sub lethal IAV infection under light anaesthesia[27]. ..

    Infection:

    Article Title: Anti-ST2 antibody reduces airway hyperresponsiveness mediated by monocyte-derived macrophages during influenza A infection.
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) of BAL and lung homogenate supernatants using mouse DuoSet ELISA kits for IL-33, IL-13, CCL2 (R&D Systems), express cysteinyl leukotrienes (CysLTs) (Cayman Chemical, Ann Arbor, MI, USA) and serotonin (Abcam, Cambridge, UK) were conducted according to the manufacturer’s instructions. .. Anti-ST2 and rat IgG2B isotype control antibodies (R&D Systems, Minneapolis, Minnesota, USA), 10 μg each in sterile PBS, were delivered intranasally a day before and a day following sub lethal IAV infection under light anaesthesia[27]. ..

    Pore Size:

    Article Title: The IKK?-dependent NF-?B p52/RelB non-canonical pathway is essential to sustain a CXCL12 autocrine loop in cells migrating in response to HMGB1
    Article Snippet: Recombinant murine SDF-1) was from PeproTech (Rocky Hill, NJ). .. Other reagents were obtained from the following suppliers: 8 μm pore size cellulose nitrate filters (for macrophages) and PVP-free polycarbonate filters (for fibroblasts) for 48 well microchemotaxis (Boyden-type) chambers (Neuroprobe Inc., Cabin John, MD); fibronectin (Roche), human recombinant PDGF (R&D Systems, Minneapolis, MN); Human recombinant complement C5a, 4-hydroxytamoxifen (4-OHT), AMD3100 (Sigma, St. Louis, MO), alexa flour 488 conjugated streptavidin (Molecular probes, Eugene, OR), alexa flour 647 conjugated anti-CXCR4 antibody (BioLegend) and biotin conjugated anti-RAGE antibody, anti-CXCR4 neutralizing monoclonal antibody, mouse IgG2a and rat IgG2b isotype control antibodies (all from R&D systems). ..

    Recombinant:

    Article Title: The IKK?-dependent NF-?B p52/RelB non-canonical pathway is essential to sustain a CXCL12 autocrine loop in cells migrating in response to HMGB1
    Article Snippet: Recombinant murine SDF-1) was from PeproTech (Rocky Hill, NJ). .. Other reagents were obtained from the following suppliers: 8 μm pore size cellulose nitrate filters (for macrophages) and PVP-free polycarbonate filters (for fibroblasts) for 48 well microchemotaxis (Boyden-type) chambers (Neuroprobe Inc., Cabin John, MD); fibronectin (Roche), human recombinant PDGF (R&D Systems, Minneapolis, MN); Human recombinant complement C5a, 4-hydroxytamoxifen (4-OHT), AMD3100 (Sigma, St. Louis, MO), alexa flour 488 conjugated streptavidin (Molecular probes, Eugene, OR), alexa flour 647 conjugated anti-CXCR4 antibody (BioLegend) and biotin conjugated anti-RAGE antibody, anti-CXCR4 neutralizing monoclonal antibody, mouse IgG2a and rat IgG2b isotype control antibodies (all from R&D systems). ..

    Incubation:

    Article Title: Longitudinal changes in the expression of IL-33 and IL-33 regulated genes in relapsing remitting MS
    Article Snippet: Cells were then washed twice with 0.75 ml of ice-cold perm/wash solution (BD Biosciences, San Diego, CA) at 3000 rpm for 5 min each. .. After washing, cells were incubated with 3 μl of PE conjugated rat (IgG2B) anti-human IL-33 (R&D systems; cat # IC3625P; Minneapolis, MN) antibodies in 150 μl volume of perm wash buffer at room temperature for 1 h. Part of the unstained and CD14 stained cells were also stained with rat (IgG2B) isotype control antibodies (R&D systems; cat # IC013P; Minneapolis, MN) in 150 μl volume of perm wash buffer at room temperature for 1 h. Cells were then washed twice with 0.75 ml of ice-cold perm/wash solution and re-suspended in 0.5 ml of PBS for flow cytometry analysis. shows the flow cytometric distribution of CD14+ IL-33+ cells. ..

    Staining:

    Article Title: Longitudinal changes in the expression of IL-33 and IL-33 regulated genes in relapsing remitting MS
    Article Snippet: Cells were then washed twice with 0.75 ml of ice-cold perm/wash solution (BD Biosciences, San Diego, CA) at 3000 rpm for 5 min each. .. After washing, cells were incubated with 3 μl of PE conjugated rat (IgG2B) anti-human IL-33 (R&D systems; cat # IC3625P; Minneapolis, MN) antibodies in 150 μl volume of perm wash buffer at room temperature for 1 h. Part of the unstained and CD14 stained cells were also stained with rat (IgG2B) isotype control antibodies (R&D systems; cat # IC013P; Minneapolis, MN) in 150 μl volume of perm wash buffer at room temperature for 1 h. Cells were then washed twice with 0.75 ml of ice-cold perm/wash solution and re-suspended in 0.5 ml of PBS for flow cytometry analysis. shows the flow cytometric distribution of CD14+ IL-33+ cells. ..

    Flow Cytometry:

    Article Title: Longitudinal changes in the expression of IL-33 and IL-33 regulated genes in relapsing remitting MS
    Article Snippet: Cells were then washed twice with 0.75 ml of ice-cold perm/wash solution (BD Biosciences, San Diego, CA) at 3000 rpm for 5 min each. .. After washing, cells were incubated with 3 μl of PE conjugated rat (IgG2B) anti-human IL-33 (R&D systems; cat # IC3625P; Minneapolis, MN) antibodies in 150 μl volume of perm wash buffer at room temperature for 1 h. Part of the unstained and CD14 stained cells were also stained with rat (IgG2B) isotype control antibodies (R&D systems; cat # IC013P; Minneapolis, MN) in 150 μl volume of perm wash buffer at room temperature for 1 h. Cells were then washed twice with 0.75 ml of ice-cold perm/wash solution and re-suspended in 0.5 ml of PBS for flow cytometry analysis. shows the flow cytometric distribution of CD14+ IL-33+ cells. ..



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    R&D Systems rat igg 2b isotype control antibody
    IL-6 signaling through the IL-6 receptor limits intracellular UPEC and IBC formation in the urothelium in vivo. a IL-6 neutralization with intraperitoneal (IP) IL-6 antibody (IL-6ab) injection in WT mice inhibits pSTAT3 by rIL-6 given IP as compared to isotype control, IgG1. b IL-6ab-treated WT mice had increased IBC formation 6 hpi with 10 8 UPEC (strain CFT073) compared to IgG1-treated mice ( p = 0.0192, Mann-Whitney U test). c Blockade of IL-6R in WT mice leads to similar inhibition of pSTAT3 by rIL-6 given IP as compared to isotype control, <t>IgG2b.</t> d Increased IBC formation at 6 hpi ( p = 0.0063, unpaired t test). e Increased intracellular UPEC recovery from IL-6 KO vs. WT bladders 6 hpi based on in vivo gentamicin protection assay ( p = 0.0364, Mann-Whitney U test). f RIL-6 given IP to IL-6 KO mice restores pSTAT3, and decreases IBC recovery compared to PBS carrier injection at 6 hpi ( g ) that is sustained at 24 hpi ( h ) (strain CFT073) ( p = 0.0118, unpaired t test and 0.0134, Mann-Whitney U test). Each dot in ( b ), ( d ), ( e ), ( g ), and ( h ) represents an individual mouse with the line at the median value.
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    Image Search Results


    IL-6 signaling through the IL-6 receptor limits intracellular UPEC and IBC formation in the urothelium in vivo. a IL-6 neutralization with intraperitoneal (IP) IL-6 antibody (IL-6ab) injection in WT mice inhibits pSTAT3 by rIL-6 given IP as compared to isotype control, IgG1. b IL-6ab-treated WT mice had increased IBC formation 6 hpi with 10 8 UPEC (strain CFT073) compared to IgG1-treated mice ( p = 0.0192, Mann-Whitney U test). c Blockade of IL-6R in WT mice leads to similar inhibition of pSTAT3 by rIL-6 given IP as compared to isotype control, IgG2b. d Increased IBC formation at 6 hpi ( p = 0.0063, unpaired t test). e Increased intracellular UPEC recovery from IL-6 KO vs. WT bladders 6 hpi based on in vivo gentamicin protection assay ( p = 0.0364, Mann-Whitney U test). f RIL-6 given IP to IL-6 KO mice restores pSTAT3, and decreases IBC recovery compared to PBS carrier injection at 6 hpi ( g ) that is sustained at 24 hpi ( h ) (strain CFT073) ( p = 0.0118, unpaired t test and 0.0134, Mann-Whitney U test). Each dot in ( b ), ( d ), ( e ), ( g ), and ( h ) represents an individual mouse with the line at the median value.

    Journal: Journal of Innate Immunity

    Article Title: Interleukin-6 Limits Host Susceptibility to Urinary Tract Infection by Promoting Urothelial Expulsion of Intracellular Bacteria

    doi: 10.1159/000550787

    Figure Lengend Snippet: IL-6 signaling through the IL-6 receptor limits intracellular UPEC and IBC formation in the urothelium in vivo. a IL-6 neutralization with intraperitoneal (IP) IL-6 antibody (IL-6ab) injection in WT mice inhibits pSTAT3 by rIL-6 given IP as compared to isotype control, IgG1. b IL-6ab-treated WT mice had increased IBC formation 6 hpi with 10 8 UPEC (strain CFT073) compared to IgG1-treated mice ( p = 0.0192, Mann-Whitney U test). c Blockade of IL-6R in WT mice leads to similar inhibition of pSTAT3 by rIL-6 given IP as compared to isotype control, IgG2b. d Increased IBC formation at 6 hpi ( p = 0.0063, unpaired t test). e Increased intracellular UPEC recovery from IL-6 KO vs. WT bladders 6 hpi based on in vivo gentamicin protection assay ( p = 0.0364, Mann-Whitney U test). f RIL-6 given IP to IL-6 KO mice restores pSTAT3, and decreases IBC recovery compared to PBS carrier injection at 6 hpi ( g ) that is sustained at 24 hpi ( h ) (strain CFT073) ( p = 0.0118, unpaired t test and 0.0134, Mann-Whitney U test). Each dot in ( b ), ( d ), ( e ), ( g ), and ( h ) represents an individual mouse with the line at the median value.

    Article Snippet: For IL-6 and IL-6R neutralization, WT mice received 200 μL of intraperitoneal injection of either 1 mg of anti-mouse IL-6 antibody (BioXCell, Lebanon NH, clone MP5-20F3) versus rat IgG1 isotype control (BioXCell, clone HRPN), or 500 μg of IL-6R neutralizing antibody (BioXCell, clone 15A7) versus rat IgG2b isotype control (BioXCell, clone LTF-2) 24 h prior to IL-6 treatment or UTI.

    Techniques: In Vivo, Neutralization, Injection, Control, MANN-WHITNEY, Inhibition